l929 mouse fibroblast cells  (ATCC)

Journal: Antioxidants

Article Title: In Vitro Evaluation of Redox-Associated Responses Induced by Mud Extract in L929 and RAW 264.7 Cells

doi: 10.3390/antiox15040448

Figure Lengend Snippet: Effects of mud extract on the viability and proliferation of L929 and RAW 264.7 cells. Left panels ( A , C ) represent L929 fibroblasts, and right panels ( B , D ) represent RAW 264.7 macrophages. ( A , B ) Cell viability was evaluated using the WST assay after treatment with indicated concentrations (10, 100, and 1000 μg/mL) of mud extract for 24 and 48 h. ( C , D ) To rule out potential optical interference from the extract, cell proliferation was orthogonally validated using the trypan blue exclusion assay via direct cell counting. The data are presented as the mean ± standard deviation (SD) of three independent experiments ( n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001. Asterisks indicate statistically significant differences compared to the respective control (Ctrl) group at each time point. Bars without asterisks indicate no significant difference ( p > 0.05) compared to the Ctrl.

Article Snippet: L929 mouse fibroblasts and RAW 264.7 mouse macrophages were obtained from the Korean Cell Line Bank (Seoul, Republic of Korea).

Techniques: WST Assay, Trypan Blue Exclusion Assay, Cell Counting, Standard Deviation, Control

Journal: Antioxidants

Article Title: In Vitro Evaluation of Redox-Associated Responses Induced by Mud Extract in L929 and RAW 264.7 Cells

doi: 10.3390/antiox15040448

Figure Lengend Snippet: Kinetic profiles of intracellular ROS generation in L929 and RAW 264.7 cells. Intracellular ROS levels were measured using the DCFH-DA assay at 1, 6, and 24 h after treatment with various concentrations (10, 100, and 1000 μg/mL) of the mud extract. The red dotted line represents the baseline level (100%) of the untreated control (Ctrl). ( A ) L929 fibroblasts and ( B ) RAW 264.7 macrophages. The data are expressed as a percentage of the untreated control (Ctrl) after background subtraction. All values represent the mean ± SD ( n = 3). Asterisks indicate significant differences compared to the control (* p < 0.05).

Article Snippet: L929 mouse fibroblasts and RAW 264.7 mouse macrophages were obtained from the Korean Cell Line Bank (Seoul, Republic of Korea).

Techniques: DCFH-DA Assay, Control

Journal: Antioxidants

Article Title: In Vitro Evaluation of Redox-Associated Responses Induced by Mud Extract in L929 and RAW 264.7 Cells

doi: 10.3390/antiox15040448

Figure Lengend Snippet: Kinetic profiles of antioxidant enzyme activities in L929 and RAW 264.7 cells. The relative activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were quantified at 1, 6, and 24 h after treatment with various concentrations (10, 100, and 1000 μg/mL) of the mud extract. Left panels ( A , C , E ) represent L929 fibroblasts, and right panels ( B , D , F ) represent RAW 264.7 macrophages. ( A , B ) SOD-associated activity was measured by monitoring the inhibition of WST-1 reduction. ( C , D ) CAT activity was determined by measuring the decomposition of H 2 O 2 through a HRP-linked colorimetric assay. ( E , F ) GPx activity was quantified by monitoring the rate of NADPH consumption at 340 nm. To eliminate potential optical interference from the mineral components of the extract, background absorbance from cell-free blanks was subtracted for each concentration across all assays. The data are expressed as a percentage of the untreated control (Ctrl) and represented as the mean ± SD ( n = 3). Asterisks indicate significant differences compared to the Ctrl (* p < 0.05, ** p < 0.01). Bars without asterisks indicate no significant difference ( p > 0.05) compared to the Ctrl.

Article Snippet: L929 mouse fibroblasts and RAW 264.7 mouse macrophages were obtained from the Korean Cell Line Bank (Seoul, Republic of Korea).

Techniques: Activity Assay, Inhibition, Colorimetric Assay, Concentration Assay, Control

Journal: Antioxidants

Article Title: In Vitro Evaluation of Redox-Associated Responses Induced by Mud Extract in L929 and RAW 264.7 Cells

doi: 10.3390/antiox15040448

Figure Lengend Snippet: Transcriptional induction of the Nrf2/HO-1 signaling pathway by mud extract. Relative mRNA expression levels of ( A ) Nrf2 and ( B ) HO-1 were determined by RT-qPCR in L929 and RAW 264.7 cells 12 h after treatment with various concentrations (0, 10, 100, and 1000 μg/mL) of mud extract. Values were normalized to GAPDH and are expressed as fold changes relative to the untreated control (0 μg/mL). While L929 fibroblasts showed a modest increase in antioxidant gene expression, RAW 264.7 macrophages exhibited a robust, dose-dependent upregulation of both Nrf2 and HO-1. The data are presented as the mean ± SD ( n = 3). Asterisks indicate statistically significant differences compared with the 0 μg/mL group (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: L929 mouse fibroblasts and RAW 264.7 mouse macrophages were obtained from the Korean Cell Line Bank (Seoul, Republic of Korea).

Techniques: Expressing, Quantitative RT-PCR, Control, Gene Expression